In vitro antiparasitic effects of six beverages on the growth of Babesia and Theileria parasites

MAHMOUD REZK ABOULAILA, MOHAMED A RIZK1, SHIMAA EL-SAYED, NAOAKI YOKO-YAMA, IKUO IGARASHI

Abstract


Introduction: To evaluate the inhibitory effects of the decoction of green tea, black tea, instant coffee, hibiscus, cinnamon, and peppermint on the in vitro growth of Babesia bovis, Babesia bigemina, Babesia caballi, Babesia divergens, and Theilria equi.

Method: In vitro inhibition assay of the decoction of green tea, black tea, instant coffee, hibiscus, cinnamon, and peppermint was used for Babesia bovis, Babesia bigemina, Babesia caballi, Babesia divergens, and Theilria equi. Viability test was applied after treatment of the cultures with beverages.

Results: In the present study, the six beverages significantly inhibit the in vitro growth of the four Babesia species and T. equi. The IC50 values of green tea were 3.83, 6.25, 2.2, 5.3, and 1.8 % (v/v) for Babesia bovis, Babesia bigemina, Babesia divergens, Babesia caballi, and Theilria equi, respectively. The IC50 values of black tea were 3.8, 10, 2.92, 12.6, and 1.9 % (v/v) for B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The IC50 values of instant coffee were 0.9, 0.5, 0.42, 4.5, and 0.75 % (v/v) for B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively.  The IC50 values of hibiscus were 16.5, 26.5, 5, 7.9, and 14.1 % (v/v) for B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The IC50 values of cinnamon were 7.83, 19, 5.9, 12.1, and 6% (v/v) for B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. The IC50 values of peppermint were 26.4, 27.5, 7.6, 26.8, and 8.54 % (v/v) for B. bovis, B. bigemina, B. divergens, B. caballi, and T. equi, respectively. 

Conclusion: The present study presented the health benefits of these natural beverages as antiprotozoal agents in vitro. Further study for their in vivo evaluation is needed.    

 


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